Figure 1.

Preparation and characterization of tissue engineering skin. (a) Photo images of computer-aided 3D-bioprinter used for 3D-printed PLCL scaffold preparation. (b) Drawn pictures of designed 3D-printed PLCL scaffold showed the structure and printing process. The designed diameter of the microfilament was 0.2 mm (black arrows), each hole was built by three microfilaments (green boxes), and the designed hole size was 1.2 × 1.2 × 0.6 mm³ (red boxes). The scaffold was layer-by-layer printed and the nozzle turned 90° every three layer (left drawn picture). (c) Photo pictures and semi-quantification result showed the morphology of 3D-printed PLCL scaffold which had 100 holes with the average of the length of the hole was 1.21 ± 0.0898 mm, the width of the hole was 1.17 ± 0.0627 mm, and the height of the hole was 0.49 ± 0.0583 mm, respectively. Bar: 2 mm.