Table 2.
Neutrality and genetic variability tests performed on each MLST locus.
| Locus | No. of alleles | Length (bp) | Total Number of Sites1 | No. of polymorphic sites (SNP) | No. of Haplotypes | dN-dS | dN/dS 2 | Nucleotide Diversity (π) | Haplotype Diversity (Hd) | Average no. of nucleotide differences (k) | Tajima’s D3 | Tajima’s D (P-value) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACT | 22 | 830 | 824 | 12 | 12 | -0.98 | <1 | 0.00367 | 0.853 | 3.02401 | 0.87646 | >0.10 |
| CAL | 8 | 689 | 689 | 5 | 8 | -0.39 | <1 | 0.00196 | 0.702 | 1.35305 | 0.63566 | >0.10 |
| EF1a | 23 | 715 | 681 | 13 | 12 | -0.12 | <1 | 0.00277 | 0.695 | 1.88838 | -0.54682 | >0.10 |
| RPB2 | 15 | 952 | 952 | 18 | 15 | -3.62 | <1 | 0.00518 | 0.864 | 4.93145 | 1.56775 | >0.10 |
| SOD2 | 18 | 437 | 433 | 18 | 16 | 0.81 | >1 | 0.01335 | 0.770 | 5.78177 | 2.29468 | <0.05 |
| TUB | 12 | 401 | 393 | 11 | 11 | -0.93 | <1 | 0.00517 | 0.582 | 2.03089 | 0.17512 | >0.10 |
| Concatenated | 159 (ST) | 4024 | 3972 | 77 | 149 | 0.00471 | 0.9965 | 18.67192 | 1.31347 | >0.10 |
1Excluding sites with gaps/missing data.
2Non synonymous-synonymous substitutions ratio determined as described by Nei and Gojobori (1986) in MEGA version 11 (Tamura et al., 2021).
3Tajima’s test for neutrality (Tajima, 1989).