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. Author manuscript; available in PMC: 2022 Jan 10.
Published in final edited form as: Insect Biochem Mol Biol. 2020 Mar 10;120:103359. doi: 10.1016/j.ibmb.2020.103359

Fig. 6. Non-target arthropods survive treatments with dop1.462 IRPs.

Fig. 6.

Consumption of dop1.462 yeast by D. pulex (A; n=60/treatment), D. magna adults (B; n=60/treatment), Tribolium castaneum larvae (C; n=120/treatment), or D. melanogaster larvae (D; n=140/treatment) had no significant impact on survival (P>0.05 with respect to arthropods treated with control IRPs). In all replicate trials conducted on these larvae (A-D), 20 individuals were fed 40 mg of yeast at the onset of the trial, which concluded when all insects had emerged as adults. Likewise, no significant differences in mortality were observed in adult D. melanogaster fed with dop1.462 ATSB, sugar bait alone, or sugar bait with control siRNA, (E; n=40/treatment; 20 adults were fed 32 μl of 2.5 μg/μl siRNA at the onset of the trial, with mortality assessed six days following treatment). Data in A-E are displayed as mean percentages of survival with error bars representing SEM. The survival data shown in A-D were compiled from multiple biological replicate trials (three in A and B, six in C, seven in D) and analyzed using the Fisher’s exact test, while survival data shown in E were compiled from two replicate experiments and analyzed using the G-test.