Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jan 10;22:50. doi: 10.1186/s12885-021-08870-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — EVs isolated from lymphatic drainage fluid from breast cancer patients by SEC isolation. EVs were isolated from lymphatic drainage fluid of seven breast cancer patients using qEV SEC. EV-containing fractions were collected, pooled, concentrated using ultrafiltration and analyzed further. A EV particle number and protein amount isolated per ml of lymphatic drainage fluid from each of the 7 patients as analyzed by nFCM and Qubit. B Size distribution of EVs from patient 1 as analyzed by nFCM. C Western shows the presence of EV proteins CD63 and Flotillin and absence of albumin in EVs isolated from all patients. All EV samples contained lipoprotein ApoA1. The positive control used for albumin was human melanoma metastatic tissue and 9–10 μg protein was loaded in each lane. Western blot membranes are cropped, uncropped membranes are shown in Additional file 7. D TEM images show vesicle-like structure in the size range of 30–200 nm in EVs isolated from the breast cancer patients. Size bar, 200 nm