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. 2022 Jan 10;41:18. doi: 10.1186/s13046-021-02203-2

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© The Author(s) 2021, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — HuD interacts with and stabilizes GRB-10 and ARL6IP1 RNA transcripts in neuroblastoma cells. A RNA targets of HuD. B HuD-GRB-10 interaction by RIP/RT-qPCR in IMR-32 and HEK293 cells (control vs. FLAG-HuD-overexpressed). C HuD-ARL6IP1 interaction by RIP/RT-qPCR in IMR-32 and HEK293 cells (control vs. FLAG-HuD-overexpressed). D GRB-10, ARL6IP1 and HDAC2 RNA stability assay in IMR-32 and SK-N-SH (comparison of control and silenced HuD). E Western blot analysis of Ago2 and HuD in IMR-32 cells (control or silenced HuD). Full-length blots are presented in Supplementary Fig. S9. F Ago2 bound GRB-10; RIP/RT-qPCR in IMR-32 (control or silenced HuD). G Ago2 occupied ARL6IP1 mRNA by RIP/RT-qPCR in IMR-32 (control or silenced HuD). H Determination of Ago2 occupied GAPDH mRNA (negative control) by RIP assay followed by RT-qPCR in IMR-32 cells (control or silenced HuD). Data are presented as mean ± SEM; t test: **p < 0.01, ***p < 0.001