Fig. 2.

Effect of acacetin on EMT in MKN45 and MGC803 cells. A The protein expression levels of EMT biomarkers (E-cadherin, N-cadherin, Vimentin, MMP-9, MMP-2 and Snail) were determined by western blot analysis. B MKN45 and MGC803 cells were treated with acacetin for 24 h. Then, the cells were fixed and labelled for E-cadherin (green) and N-cadherin (red), and the nuclei were stained with DAPI (blue). The third parts show the merged images. Data are expressed as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01 compared to the vehicle control