Fig. 3.

Effect of acacetin on invasion and migration in TGF-β1 (10 ng/ml)-treated MKN45 and MGC803 cells. A Morphological changes in MKN45 and MGC803 cells that were untreated or treated with acacetin. B Wound healing assays detected the effect of acacetin on TGF-β1 (10 ng/ml)-induced invasion of GC cells. C Transwell assays measured invasion and migration influenced by acacetin in GC cells under TGF-β1 treatment. D Western blot analysis of the protein changes in TGF-β1-induced EMT in GC cells. The quantitative values are expressed as the mean ± SD (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 compared to the control group. # p < 0.05, ## p < 0.01, ### p < 0.001 compared to the TGF-β1 alone group