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. 2022 Jan 10;23:42. doi: 10.1186/s12864-021-08261-2

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© The Author(s) 2022, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Comparison of Sequin spike-in sensitivity of detection between ONT (long read) and short read sequencing (Illumina paired-end, SRS) sequencing for Sequin synthetic spike-ins MixA (A) and MixB (B). Labelled sequin (red point) in each plot is at ONT limit of quantification (LOQ) in each mix; mix A 0.059 attomol/μl and mix B 0.27 attomol/μl. Plot C shows correlation of ONT differential isoform observed vs observed expression (log2 fold change + 1) of synthetic Sequin spike-ins of known concentration. Pearson correlation coefficient is displayed along with a linear regression trend line with standard error in pale grey