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. 2022 Jan 10;23:42. doi: 10.1186/s12864-021-08261-2

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© The Author(s) 2022, corrected publication 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Panel of A gene-level differential expression (DE) smear plot, solid red lines highlighting ±1.5 logFC threshold, B gene-level DE of CACNA2D2 during differentiation, C isoform-level DE smear plot with ±1.5 logFC threshold, D CACNA2D2 isoform expression, showing novel TALON isoform with highest read count. Red points on smear plots indicate significant differential expression (FDR < 0.05). Boxplots display median and IQR. Short and long read mapping example provided in Fig. S8