Fig. 5.

Frequency of in vivo hematopoietic progenitors in adult wild-type and Emilin-2 null mice. BM were isolated from wild-type and Emilin2^−/− mice of 3, 6 and 10 months of age and subjected to flow cytometry with different antibodies. A Representative flow cytometry analysis of hematopoietic progenitors. Sca1⁻/c-Kit⁺ events (plot iii of Fig. 4A) were subjected to further analysis, based on CD16/32 and CD34 signals, to identify GMP (CD16/32⁺/CD34⁺; top square), CMP (CD16/32⁻/CD34⁺; middle square) and MEP (CD16/32⁻/CD34⁻; bottom square). B Percentages of CMP, GMP and MEP in Lin⁻ subpopulation and percentages of Lin⁻ cells on total live cells in BM of 3-month-old (wild type, n = 4; Emilin2^−/−, n = 4). C Percentages of CMP, GMP and MEP in Lin⁻ subpopulation and percentages of Lin⁻ cells on total live cells in BM of 6-month-old (wild-type, n = 10; Emilin2^−/−, n = 6) and 10-month-old (wild type, n = 5–7; Emilin2^−/−, n = 5–9) mice. WT, wild-type. See also Additional file 1: Fig. S4