Fig. 3.

MiR-7-5p targeted gene prediction and the validation. A Seven network data bases forecast the number of targeted genes of miR-7-5p and take their intersection using Venn diagram. (B) A network map between 11 target genes and miR-7-5p was predicted. C qRT-PCR was employed to validate the expressing levels of 11 predicted targeted genes in AML patients and NC. *p < 0.05 in contrast to the NC, **p < 0.01 in contrast to the NC, ***p < 0.001 in contrast to the NC. D Expression of OSBPL11 mRNA in AML cells and human hematopoietic normal cells GM12878 by qRT-PCR. E The sequencing result of mankind miR-7-5p and the forecasted binding areas with miR-7-5p in the OSBPL11 non-translated area (3′-UTR) are displayed. F After co-culturing with MOLM13 and HL-60 cells with miR-7-5p mimics for 24 h, respectively, OSBPL11 protein expression levels were measured by immunoblotting. The quantitation data from immunoblotting analysis were analyzed via ImageJ program. G MiR-7-5p mimics were co-cultured with MOLM13 and HL-60 cells for 24 h, and PI3K, p-PI3K, AKT, p-AKT, mTOR and p-mTOR protein expressing status were identified by western blotting, respectively. The quantitation data from immunoblotting analysis were analyzed via ImageJ program, which were described as mean ± SD (n = 3)