FIG. 3.

Expanded targeting observed when using closely spaced gRNAs. (A) The location of the two gRNAs in blue are shown on the GAL4 sequence. Residues changed by the BE2 base editor are highlighted in red. Example wild-type and base-edited chromatographs from three different GAL4 fly lines are shown. Base editing occurred primarily outside the gRNA target. (B) The EditR program was used to calculate the percentage of base-editing occurring for each base in the boxed region in (A). (C) Base-editing occurring between three additional genomic loci targeted by two closely spaced gRNAs. The residues found to be mutated are in red. The number under the residue indicates the estimated percentage of conversion at that residue as calculated by EditR. Color images are available online.