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. 2021 Nov 24;15:711750. doi: 10.3389/fnsys.2021.711750

Figure 6.

Figure 6

Ex4’s treatment 0 h after each extinction attenuates reinstatement of cocaine-induced CPP. (A) The experimental schedule for confined extinction procedures and saline (Sal), cocaine (Coc), as well as exendin4 (Ex4) treatment protocals. (B) CPP scores of pre-test and post-test indicate that most animals showed a significant place preference following cocaine-associated CPP training. N = 13 per group, **represents p < 0.01, paired t-test. (C) CPP scores after systemic administration of vehicle and Ex4 show that Ex4attenuated reinstatement. N = 5 per group, **represents p < 0.01 vs. pretest, ns represents no significance, two-way ANOVA. (D) Distance traveled of vehicle and Ex4 on the cocaine-primed reinstatement test day. ns represents no significance vs. vehicle group, Student’s t-test. (E) Representative image of TLR4 expression in the saline, vehicle and Ex4 groups detected by Western blot analysis. (F) Semiquantitative analysis of the relative levels of TLR4 in hippocampus by densitometric analysis. N = 5 per group, ***represents p < 0.001 vs. Sal group; ##represents p < 0.01 vs. vehicle group, one-way ANOVA followed by Bonferroni’s post hoc test. Date are expressed as mean ± SEM.