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. Author manuscript; available in PMC: 2022 Jan 10.
Published in final edited form as: Nature. 2021 Sep 22;598(7880):321–326. doi: 10.1038/s41586-021-03948-8

Extended Data Figure 1 |. Histological validation of implanted sites.

Extended Data Figure 1 |

(a) Optic fiber positions in the LEC of Sim1-Cre mice injected with AAV-flex-Jaws-GFP for inhibition experiments. Arrowhead, the tip of optic fibers. D, dorsal, V, ventral, M, medial, L, lateral.

(b) Optic fiber positions in the LEC of Wfs1-Cre mice for inhibition experiment of Wfs1-expresing pyramidal cells.

(c) Recording position in the superficial layer of LEC from Sim1-Cre mice for opt-tagging experiment. Note large lesions because of the electrical lesioning.

(d) Optic fiber positions in the LEC of DAT-Cre mice injected with AAV-flex-Jaws-GFP in the VTA/SNc for inhibition experiment.

(e) Optic fiber positions in the LEC of DAT-Cre x Ai14 mice for photometry experiment. Two mice received unilateral implantations and four mice received bilateral implantations.

(f) Recording position in the superficial layer of LEC from Sim1-Cre x DAT-Cre mice for opt-tagging + inhibition experiment.

(g) Optic fiber positions in the LEC of Sim1-Cre mice injected with AAV-flex-GFP for control inhibition experiment.

(h) Optic fiber positions in the LEC of Wfs1-Cre mice injected with AAV-flex-GFP for control inhibition experiment.

(i) Optic fiber positions in the LEC of DAT-Cre mice injected with AAV-flex-GFP at VTA and SNc for control inhibition experiment.

(j) Recording position in the superficial layer of LEC from Sim1-Cre x DAT-Cre mice for opt-tagging (ChR2-mCherry) + control inhibition (GFP) experiment.