Figure 12.

Representative flow cytograms were obtained by double staining Annexin V (FITC) and propidium iodide (PI). Characteristic time and dose-dependent shift of HeLa cells population from An⁻PI⁻ (lower left quadrant) to An⁺PI⁺ (upper right quadrant) progress through An⁺PI⁻ state (lower right quadrant) at each cytogram, respectively. Studied concentrations were related to IC₅₀ result of 24 h cytotoxicity assay (1/2 IC₅₀–2.83 µg/mL, IC₅₀–5.66 µg/mL, 2× IC₅₀–11.32 µg/mL, DMSO–0.4%).