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. 2022 Jan;32(1):111–123. doi: 10.1101/gr.275750.121

Figure 3.

Figure 3.

Mediator association persists at non-RP genes repressed by heat shock. (A) Heat maps and line graphs showing normalized occupancy of Pol II and Med15 at non-RP UAS genes (see text) having Pol II occupancy decreased by at least twofold upon heat shock (“UAS down”) (Supplemental Table S2) or having Pol II occupancy unchanged or increased upon heat shock (“UAS not down”) (Supplemental Table S2) in rapamycin-treated YFR1321, the parent strain to kin28AA yeast, with and without heat shock. (B) Heat maps and line graphs showing normalized occupancy of Med15 (tail) and Med18 (head) at “UAS down” and “UAS not down” genes in kin28AA yeast treated with rapamycin, with and without heat shock. (C) Browser scans showing Med15 and Pol II occupancy at ILV1 and TEF1 genes in kin28AA yeast (“−Kin28”) or the parent strain YFR1321 (“+Kin28”), both treated with rapamycin, with or without heat shock. Scale, in reads per million mapped reads, is indicated for each scan. Note that Pol II occupancy is reduced at both genes upon heat shock; the vertical dashed lines emphasize the shift of the Med15 peak toward the promoters only when Kin28 is depleted in the absence of heat shock.