Table 1.
Sec-tRNA[Ser]Sec isoforms in cultured mammalian cells.
| Sec tRNA[Ser]Sec | |||||||
|---|---|---|---|---|---|---|---|
| mcm5U | mcm5Um | ||||||
| Cell Line | Selenium Supplementation a |
% of Total b | % | % of Total c | % | % of Total d | mcm5Um/ mcm5U e |
| HL-60 | +(chem. defined media) | 9.6 | 38.5 | 3.70 | 61.5 | 5.90 | 1.60 |
| −(chem. defined media) | 7.5 | 61.3 | 4.60 | 38.7 | 2.90 | 0.63 | |
| HL-60 | +(FBS) | 9.4 | 55.3 | 5.20 | 44.7 | 4.20 | 0.81 |
| −(FBS) | 7.4 | 77.0 | 5.70 | 23.0 | 1.70 | 0.30 | |
| CHO | +(FBS) | 1.01 | 45.1 | 0.46 | 54.9 | 0.55 | 1.22 |
| −(FBS) | 0.86 | 56.2 | 0.48 | 43.8 | 0.38 | 0.78 | |
| RMT | +(chem. defined media) | 1.7 | 11.8 | 0.20 | 88.2 | 1.50 | 7.47 |
| −(chem. defined media) | 1.4 | 35.7 | 0.50 | 64.3 | 0.90 | 1.80 | |
a FBS: fetal bovine serum; b percentage of tRNA[Ser]Sec population within total Ser-tRNA population; c percentages of mcm5U and mcm5Um isoforms within total tRNA[Ser]Sec population; d percentages of mcm5U or mcm5Um isoforms within total Ser-tRNA population; e amount of mcm5Um/amount of mcm5U isoforms.