Table 4.

Mouse models involving Trsp knockout (KO) or Trsp conditional KO mice rescued with wild-type (WT), G37 mutant, or A34 transgenes.

Target Site	Model Description	Major Findings Observed in Genetically Altered Mice in Comparison to Control Mice
Whole Mouse	Trsp KO rescued with WT Trsp transgene	Selenoprotein synthesis was completely recovered [125].
	Trsp KO rescued with G37 Trsp transgene	Proper base modification in the anticodon is essential, as mutant mice synthesize stress-related selenoproteins very poorly. Male mutant mice show abnormal sperm and reduced fertility; females produced reduced litter size [43]. Trsp KO could not be rescued with A34 mutant transgene most likely due to misreading (see Text).
Whole Mouse	Trsp KO rescued with promoter mutant Trsp transgene	Mice expressed tissue- and organ-specific amounts of tRNA[Ser]Sec. Lower levels of the mcm5Um isoform were observed in promoter mutant Trsp mice. Mice developed a similar neurological phenotype as SELENOP-KO mice and a reduced life span [157].
Liver Alb-Cre	Trsp liver KO rescued with Trsp WT transgene	Selenoprotein synthesis was completely recovered [82].
	Trsp liver KO rescued with G37 mutant Trsp transgene	Housekeeping selenoprotein synthesis was recovered while stress-related selenoprotein synthesis was poorly recovered [82].
	Trsp liver KO rescued with A34 mutant Trsp transgene	Housekeeping selenoprotein synthesis was recovered while stress-related selenoprotein synthesis was poorly recovered. Replacement of selenoprotein synthesis in conditional Trsp mutants resulted in normal gene expression of Phase II response enzymes [127,145].