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. 2021 Dec 21;23(1):58. doi: 10.3390/ijms23010058

Figure 4.

Figure 4

Linc013026-68AA enhances cell proliferation. (A) HeLa cells were transfected with Myc-tagged Linc013026-68AA (68AA-Myc) and pcDNA3.1 MycHis (Vector) vector for two days and anti-Linc013026-68AA- and Actin-specific immunoblot were performed. ACTIN was used as loading control. (B,C) Sister cultures of (A) were supplied for crystal violet- (B) and Wst-1 assay (C) to examine the effect of Linc013026-68AA on cell proliferation. (D,E) HepG2 cells were transfected with siRNA control (siCr) and siLinc013026 (si68AA) for three days and supplied for crystal violet- (D) and Wst-1 assay (E). (F) Total RNAs were isolated from HeLa, Huh7, HLE, HepG2, C3A and Hep3B cells. The expression of Linc013026 was examined using RT-PCR. Gapdh mRNA was used as loading control. [] indicates number of PCR cycles. (G) Huh7- and HepG2 cells were transfected with Myc-tagged Linc013026-68AA (68AA-Myc) and pcDNA3.1 MycHis (Vector) vector for two days and supplied for crystal violet- and Wst-1 assay. Three independent experiments were performed for crystal violet- and Wst-1 assay. Numbers are mean ± standard deviation (SD). p: p-value.