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. Author manuscript; available in PMC: 2022 Jan 10.
Published in final edited form as: HNO. 2020 Feb;68(2):100–105. doi: 10.1007/s00106-019-00811-1

Figure 2.

Figure 2.

Specificity of anti-CSPG4 mAb clones TP4 and TP6, which recognize distinct epitopes of CSPG4 antigen, for detection of this antigen on MTEX and nonMTEX. In A, nearly all MTEX immunocaptured with clone TP4 mAb and detected with clone TP6 mAb were CSPG4(+). NonMTEX were negative for CSPG4. In B, nearly all MTEX immunocaptured with clone TP6 and detected with clone TP4 mAb were CSPG4(+). NonMTEX were negative for CSPG4. In A and B, the CSPG4-specific capture mAbs were used at the protein concentration of 3ug, while anti-CD63 mAb for capture of nonMTEX was 2ug. Note that dilutions of capture and detection mAbs are critical for the success of capture as well as detection of CSPG4+ exosomes.