Table 1.
Feature | Fiber Photometry | Single-photon Imaging | Two-photon Imaging |
---|---|---|---|
Resolution | Population dynamics, no single-cell resolution | Cellular resolution, overlapping cells in Z-space | Subcellular resolution, optical sectioning |
Restraint | Freely moving | Freely moving | Typically head-fixed; freely moving not widely available |
Motion Correction | Second channel used to remove motion artifacts | Required | Required |
Skill required | + | ++ | +++ |
Footprint | Small | Moderate, dual-site imaging possible | Large |
Cost | $ | $$ − $$$ | $$$$ |