Skip to main content
. 2021 Dec 28;23(1):297. doi: 10.3390/ijms23010297

Figure 1.

Figure 1

Expression of BIRC3 correlates with stem cell markers expression and self-renewal in both human and mouse GBM cells (A). Protein expression of BIRC3 in U251 and U87 GBM cells. Each cell line includes control, BIRC3 overexpression (BIRC3-OE) and BIRC3 knockout (BIRC3-KO) groups. Specific antibodies as indicated. β-actin acts as internal control. (B,C). Control, BIRC3-OE or BIRC3-KO of U251 and U87 cells were seeded in 6 well plates and cultured in neurosphere formation medium. The number of neurospheres were observed and calculated under microscope. (B): Representative images are under 4× magnification (top raw) and 20× magnification (bottom raw). (C): Representative images are under 4× magnification (top raw) and 10× magnification (bottom raw). n = 5, * p < 0.05. (D). CD133 and ABCG2 mRNA expression analyzed by real-time PCR in U251/U87 control, BIRC3-OE and BIRC3-KO cells. n = 3, * p < 0.05. (E). Immunofluorescence staining of Nestin in U251/U87 BIRC3-OE and BIRC3-KO cells. Blue: DAPI; Green: Nestin. (F). Protein expression of mBIRC3 in CT-2A mouse GBM cells including control, BIRC3-OE and BIRC3-KO groups. Specific antibodies as indicated. β-actin acts as internal control. (G). Control, BIRC3-OE or BIRC3-KO of CT-2A cells were seeded in 6 well plates and cultured in neurosphere formation medium. The number of neurospheres were observed and calculated under microscope. Representative images are under 10× magnification. n = 5, * p < 0.05. (H). Mouse CD133 and ABCG2 mRNA expression were analyzed by real-time PCR in CT-2A cells. n = 3, * p < 0.05.