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. 2021 Dec 29;23(1):340. doi: 10.3390/ijms23010340

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Representative immunofluorescence staining of Myh11⁺ cells, expressing (a) cleaved caspase 1, CD68 and (b) IL-1β, CD68 in human atherosclerotic plaques associated with NLRP3-inflammasome activation in VSMC and linked to plaque rupture in human carotid artery disease shown by confocal microscopy. Graph bars show the mean ± SEM of (c) Myh11⁺ CD68⁺ cleaved caspase 1 ⁺ and (d) Myh11⁺ CD68⁺ IL-1β⁺ co-expressing cells as a percentage of plaque Myh11 cells⁺ cells with n = 12/group and * p < 0.05, unpaired t-test.