Figure 4.

High expression of EDEM1 does not affect APP transcription in HEK293 cells. Relative APP mRNA level in HEK293 cells expressing EDEM1 was quantitatively assessed by real-time RT-PCR. Measurements were performed with the use of the Roche Universal Probe Library (UPL) assays. Expression of GAPDH mRNAs was used as reference control. Values obtained for cells without EDEM1 overexpression are indicated as 100%. Values representing EDEM1-transfected cells are plotted relative to 100%. The values are expressed as mean ± SD, n = 3, Student’s t test.