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. 2022 Jan 1;23(1):491. doi: 10.3390/ijms23010491

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Histological analysis of the ectopic bone occurrence in GCs, GCsGp and GCsGp/GO 0.5% wt.% Scaffolds 28 days post-implantation. (a) Representative H&E, Gömöri trichrome and ARS stainings. Scale Bar 20 µm; (b) The analysis of the area of collagen domains according to Gömöri staining indicated that significantly more collagen was secreted within GCsGp/GO 0.5% wt.% Group as opposed to GCs group (* p < 0.001); (c). ARS staining indicates that significantly more calcium mineral deposits are present in the GCsGp/GO 0.5% wt.% group than GCs group (* p < 0.001).