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. 2021 Dec 23;23(1):130. doi: 10.3390/ijms23010130
CA carbonic anhydrase
FTSA fluorescent thermal shift assay (or differential scanning fluorimetry, DSF)
intr intrinsic
ITC isothermal titration calorimetry
obs observed
VARIABLES
ΔGintr change of the intrinsic standard Gibbs energy upon binding
ΔGobs change of the observed standard Gibbs energy upon binding
ΔHintr change of the intrinsic standard enthalpy upon binding
ΔHobs change of the observed standard enthalpy upon binding
Kd_intr the intrinsic equilibrium dissociation constant
Kd_obs the observed equilibrium dissociation constant
Tm melting temperature (midpoint of the unfolding transition)
TΔSintr change of the intrinsic standard entropy upon binding multiplied by absolute temperature