| CA | carbonic anhydrase |
| FTSA | fluorescent thermal shift assay (or differential scanning fluorimetry, DSF) |
| intr | intrinsic |
| ITC | isothermal titration calorimetry |
| obs | observed |
| VARIABLES | |
| ΔGintr | change of the intrinsic standard Gibbs energy upon binding |
| ΔGobs | change of the observed standard Gibbs energy upon binding |
| ΔHintr | change of the intrinsic standard enthalpy upon binding |
| ΔHobs | change of the observed standard enthalpy upon binding |
| Kd_intr | the intrinsic equilibrium dissociation constant |
| Kd_obs | the observed equilibrium dissociation constant |
| Tm | melting temperature (midpoint of the unfolding transition) |
| TΔSintr | change of the intrinsic standard entropy upon binding multiplied by absolute temperature |