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. 2021 Dec 25;23(1):225. doi: 10.3390/ijms23010225

Figure 1.

Figure 1

LA induced dormancy in CT26 cells. (A) Effect of short-term LA treatment on cell proliferation. (B) Effect of continuous LA treatment up to 25 days. (Insert) Cell cycle analysis of CT26 cells with or without continuous LA treatment. (C) Effect of LA on the growth of CT26-derived subcutaneous tumors in mice. Mice were fed a CE-2 standard diet containing LA (C, standard diet; 2%, 5%, and 10%, standard diet with LA (% w/w)). (D) Subcutaneous CT26 cell aggregation (red arrow). Hematoxylin and eosin staining. (E) Immunohistochemical analysis of proliferation (Ki-67), angiogenesis (CD34), and immune cell infiltration (LCA) of CT26 tumors in 10% LA-fed or standard diet-fed mice. (F) PGE2 concentration in CT26 tumors in 10% LA-fed or standard diet-fed mice. Scale bar, 100 μm. Error bar, standard deviation based on three independent trials. LA, linoleic acid; HPF, high-power field of view; LCA, lymphocyte common antigen; PGE2, prostaglandin E2.