Figure 4.

miR-494 mediated LA-induced growth impairment in a mouse model. (A,C) Effect of miR-494 mimic (miR-494Mi) or miR-494 inhibitor (miR-494I) on LA-induced growth inhibition. CT26 cells (1 × 10⁷) were subcutaneously inoculated into BALB/c mice, which were fed a control or 10% LA diet. (Insert) Example image of excised tumors. Cont, Cont+LA, Cont+Mi or I, from the top. (B,D) Effect of miR-494 mimic (miR-494Mi) or miR-494 inhibitor (miR-494I) on LA-induced growth delay. LA-pretreated (100 μg/mL for 48 h) CT26 cells (1 × 10⁷) were subcutaneously inoculated into BALB/c mice, which were fed a control or 10% LA diet. (Insert) Example images of excised tumors. PreTx, PreTX+Mi or I, PreTx+LA, from the top. (E) Expression of miR-494, Pgc-1α, and MycC in each experimental group at week 4. U9 and Actb expression were analyzed as loading controls. miR-494 mimic and miR-494 inhibitor were administered at a dosage of 7 mg/kg body weight intraperitoneally twice per week. (F) Expression of miR-494, Pgc-1α, and MycC in each experimental group at week 4 examined by quantitative RT-PCR. Error bar, standard deviations of data from 5 mice. Scale bar, 5 mm. LA, linoleic acid; PGC, peroxisome proliferator-activated receptor gamma coactivator; ACTB, actin β.