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. 2021 Dec 29;23(1):355. doi: 10.3390/ijms23010355

Figure 2.

Figure 2

Effects of Ibrutinib post-treatment on levels of CD138 and total IgG. analyzed by quantification of Western blotting data. Western blot analysis of spinal cord samples (60 μg of protein extract each sample) at lesion epicenter showed that contusion injury increased CD138 (A,B) and total IgG (C,D) in the spinal cord 7 (A,C) and 14 (B,D) days post-injury compared with sham-operated animals. Ibrutinib treatment (6 mg/kg/day, starting at 3 h post-injury for 7 days) resulted in reduced levels of CD138 (A,B) and total IgG (C,D) in the spinal cord lesion site at 7 (A,C) and 14 (B,D) days after contusive SCI compared with vehicle-treated animals. Quantification of CD138/GADPH and total IgG/GAPDH 7 and 14 days after contusive SCI was performed by the fold of blot density. Data are presented as mean ± S.E.M., n = 4 per group (7-day time point) or n = 2 per group (14-day time point), and analyzed with one-way ANOVA followed by Bonferroni post hoc analysis, * p < 0.05, ** p < 0.01, *** p < 0.001, compared to vehicle-treated SCI animals.