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Ibrutinib did not reduce CD45RA-positive B cell population after SCI Injury. Flow cytometry analysis of splenic samples showed that there were no differences in CD45RA-positive B cell population between Ibrutinib-treated and vehicle-treated injured or sham-operated animals at 1-week post-injury. Anti-CD45RA-PE antibody was specific for the rat CD45RA-B cells. Data are presented as mean ± S.E.M., n = 4 per group, and analyzed with one-way ANOVA followed by Bonferroni post hoc analysis.
