Figure 8.

Effects of the 2-week treatment with Ibrutinib on lesion epicenter and spread of tissue damage after Scheme 2. Two-week treatment with Ibrutinib resulted in significant increases in tissue-sparing at the epicenter, 1, 2, & 3 mm rostral to the injury epicenter, and 1 mm caudal to the lesion epicenter (A), white matter sparing at lesion epicenter and 1 mm caudal to the lesion epicenter (B), and gray matter sparing at epicenter, 2 mm, 1 mm rostral and 1 mm caudal to the lesion epicenter (C) at 11 weeks following contusion injury to the spinal cord (T10, 180 kdyn). Data were presented as mean ± SEM and analyzed with repeated measures ANOVA followed by Bonferroni post-hoc analysis, * p < 0.05, ** p < 0.01, and *** p < 0.001, compared with vehicle treatment, n = 5/group. Bottom Panel: Photomicrographs of representative transverse spinal cord sections from rats at 11 weeks after contusive SCI (T10, 180 kdyn). The sections were from the lesion epicenter, obtained from a Sham (left), vehicle-treated injured rat (middle), and Ibrutinib (6 mg/kg/day)-treated injured rat (right). The sections were stained with eriochrome cyanine for myelin. Scale bar: 100 μm.