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Effects of different concentrations of BDNF on (A) IL-1β, (B) IL-6, and (C) TNF-α secretion in human monocyte-derived macrophages. U937 cells were transfected with CRISPR-Cas9 plasmid containing gRNA that targets p75NTR or control CRISPR-Cas9 plasmid as the control group. The cell surface expression (D) p75NTR and (E) TrkB on U937 cells were analyzed by Western blot analysis (ns, not significant; * p < 0.05; *** p < 0.001).
