Figure 1.

Gating Strategy. Overlapping events were ruled out from the FSC-H/FSC-A analysis. Subsequently, the populations were selected by size and complexity. From the lymphocyte region, T lymphocytes were identified as CD3 + and CD4 + cells, and CD19+ for B lymphocytes. Monocytes were identified as CD14+ cells. Once the three populations had been selected, the positive cells for CD74, CD44, CXCR2, CXCR4 and CXCR7 receptors were identified from the pre-established FMOs (CD44 are represented in CD4+ T lymphocytes, CD74 in B lymphocytes, CD44 in monocytes and CXCR2 in granulocytes). FSC-H/FSC-A; FSC-Height (FSC-H) by FSC-Area (FSC-A). FMO; Fluoresence minus one.