Table 1.
Peptide Name | Amino Acid Sequence | Theoretical Average Mr | Experimental Mr |
---|---|---|---|
TAMRA 1-KKAK-2mer | * KKAKASASKKAKTT | 1859.2 | 1859.7 |
TAMRA-KKAK-3mer | * KKAKASASKKAKASASKKAKTT | 2631.1 | 2631.8 |
TAMRA-KKQK-2mer | * KKQKQSQSKKQKTT | 2087.4 | 2087.3 |
TAMRA-KKQK-3mer | * KKQKQSQSKKQKQSQSKKQKTT | 3030.4 | 3030.9 |
TAMRA-KKQKASAS-2mer | * KKQKASASKKQKTT | 1973.3 | 1973.1 |
TAMRA-KKQKASAS-3mer | * KKQKASASKKQKASASKKQKTT | 2802.2 | 2802.4 |
TAMRA-KKKQQK-2mer | * KKKQQKQSQSKKKQQKTT | 2600.0 | 2599.3 |
TAMRA-CXCL9(74-103) | * KKKQKNGKKHQKKKVLKVRKSQRSRQKKTT | 4072.8 | 4072.9 |
1 The produced peptides were labeled with 5(6)-carboxytetramethylrhodamine (TAMRA) at the NH2-terminus (indicated in pink [*]). Glycosaminoglycan (GAG)-binding motifs are underlined.