Figure 6.

Senescence measurements in SAOS and SAOS400 cell lines. SA-βGal staining in SAOS and SAOS400 cells before and after irradiation (5 Gy) were quantified (panel a) and microscopically visualized (panel b). The results of a different protocol that employed C₁₂FDG staining were also quantified (panel c), and representative images were reproduced (d). Bar graphs represent the mean ± SD of two independent experiments. Symbols indicate significance: +++ p < 0.001 for untreated SAOS vs. untreated SAOS400 cells; *** p < 0.001 for untreated SAOS vs. irradiated SAOS (black bars); n.s. not significant in SAOS400 before and after irradiation (red bars) One-way ANOVA with Bonferroni’s multiple comparisons test was used. Panel (e) reports the immunoblot showing the basal expression of p16^INK4 in SAOS and SAOS400 cells. Numbers between panels indicate the densitometric analysis ± SD of three independent experiments. Symbols indicate significance: ** p < 0.01 vs. SAOS (T-Test Student).