Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Dec 28;23(1):301. doi: 10.3390/ijms23010301

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Senescence measurements in HT29 and HT500 cell lines. SA-βGal staining in HT29 and HT500 cells before and after irradiation (5 Gy) were quantified (panel a) and microscopically visualized (panel b). The results of a different protocol that employed C₁₂FDG staining were also quantified (panel c), and representative images were reproduced (d). Bar graphs represent the mean ± SD of two independent experiments. Symbols indicate significance: * p < 0.05 for untreated HT29 vs. irradiated HT29 (grey bars); §§§ p ≤ 0.001 for HT500 before and after irradiation (dark red bars) One-way ANOVA with Bonferroni’s multiple comparisons test was used. The immunoblots report the basal expression of p16^INK4 (panel e) and p21^CIP1 (panel f) in HT29 and HT500 cells. Numbers between panels indicate densitometric analysis ± SD of two independent experiments. Symbols indicate significance with * p < 0.05 and *** p < 0.001 (t-test Student).