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. 2021 Dec 28;23(1):301. doi: 10.3390/ijms23010301

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Changes in senescence markers following senolytic treatments in SAOS400 and HT500 cell lines. Immunoblot analysis of p16^INK4 expression in SAOS400 cells (panel a) pre-irradiated (10 Gy) and treated with F (40 μM) or Q (40 μM) for 48 h. Densitometric analysis (numbers between panels) was obtained normalizing the expression of p16^INK4 with α−tubulin and quantified as described in the Methods section. * p < 0.05 vs. Q and IR; ** p < 0.01 vs. F and IR. Immunoblots of p16^INK4 (panel b) and p21^CIP1 (panel c) expressions in HT500 cells pre-irradiated (10 Gy) and treated with 40 μM F or 40 μM Q for 72 h. Densitometric analyses (numbers between panels) were obtained normalizing the expressions of p21^CIP1 and p16^INK4 with α-tubulin and quantified as described Methods section. § p < 0.05 vs. untreated; §§ p < 0.01 vs. untreated; * p < 0.05, ** p < 0.01 vs. F plus Q and IR.

Changes in senescence markers following senolytic treatments in SAOS400 and HT500 cell lines. Immunoblot analysis of p16^INK4 expression in SAOS400 cells (panel a) pre-irradiated (10 Gy) and treated with F (40 μM) or Q (40 μM) for 48 h. Densitometric analysis (numbers between panels) was obtained normalizing the expression of p16^INK4 with α−tubulin and quantified as described in the Methods section. * p < 0.05 vs. Q and IR; ** p < 0.01 vs. F and IR. Immunoblots of p16^INK4 (panel b) and p21^CIP1 (panel c) expressions in HT500 cells pre-irradiated (10 Gy) and treated with 40 μM F or 40 μM Q for 72 h. Densitometric analyses (numbers between panels) were obtained normalizing the expressions of p21^CIP1 and p16^INK4 with α-tubulin and quantified as described Methods section. § p < 0.05 vs. untreated; §§ p < 0.01 vs. untreated; * p < 0.05, ** p < 0.01 vs. F plus Q and IR.