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. 20 CPER-derived viruses mirror infection kinetics of the respective parental clinical isolates in human cell cultures. Huh7.5 cells were infected with the parental clinical isolates and CPER-derived viruses at an MOI of 0.1. Intracellular viral RNA (A) was determined at the indicated timepoints by RT-qPCR, and viral titers (B) were quantified by a focus-forming assay at 4 days post infection. Statistical significance was assessed using Student’s t test (Original vs CPER).
