Figure 5.

. Infection of humanized mice with DENV results in activation of T cells and immune responses. (A) Representative flow cytometry plots of CD38/HLA-DR gating, along with associated phenotypes. CD38 and HLA-DR expression was assessed among gated human CD8⁺ T-cells, and subsets were characterized as naïve (CD38^– HLA-DR^–) or activated (CD38⁺ HLA-DR⁺) [43]. Frequencies of the activated subpopulation (CD38⁺ HLA-DR⁺) within CD8⁺ T cells in the plasma (B) are shown. All data is reported as frequencies of total CD45⁺ cells. Human DENV-specific IgM (C) IgG (D) antibodies circulating in sera of HIS-NFA2/hFLT3Lg mice infected with DENV. Both antibody levels were assessed using ELISA at days 0, 5, 10, 15, and 20 post-infection. Symbols represent individual mice and the median presented as a line per subset. Statistical significance was assessed using Friedman with Dunnett’s test (B, C, and D) and is indicated by asterisks (*, compared between data at day 0 or control cohort).