Fig. 1.

TH treatment regime and tissue collection in TH-deficient mice. Mice were injected on days 5–14 and tissues collected on day 21. Beginning at 5 days after birth, TH-deficient Tshr^−/− mice were injected with T3/T4, TH receptor (TR) β-specific agonist GC-1, or vehicle control (NaOH) daily for 10 days. Euthyroid control mice (Tshr^+/− littermates) were injected with vehicle control (NaOH) daily for 10 days. At postnatal day 14, injections were stopped, and all mice received standard laboratory care until postnatal day 21, at which time mice were euthanized and tissues were collected for analysis