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. 2022 Jan 4;23(1):551. doi: 10.3390/ijms23010551

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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mRNA expression levels (mean and standard error) of (A) BAX, (B) HSP70 (C) HO-1, and (D) TRNRD-1, which are related to stress conditions, in J774 cells. The amount of mRNA of each gene for each experiment was normalized with the respective value of the beta-actin housekeeping gene mRNA (X-fold of beta-actin expression). Statistical comparison of the gene’s expression in different experimental conditions was performed with 5.0 GraphPad Prism software. The data were also analyzed with one-way ANOVA. p values < 0.05 was considered significant.