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. 2021 Dec 31;23(1):450. doi: 10.3390/ijms23010450

Figure 1.

Figure 1

Paclitaxel-resistant HT1197 cells show β-catenin overexpression and CSC-like properties. (A) Western blots of PARP, cleaved caspase-3, total β-catenin, active β-catenin and p-GSK3βSer9 in 5637 and HT1197 cells treated with DMSO (vehicle) or 0.1 µM paclitaxel for 48 h, and (B) Western blots of CSC markers ALDH1A1, Sox-2 and Oct-4 in untreated 5637 and HT1197 cells. β-actin was used as a loading control. Histograms show the densitometric analysis of the indicated proteins. Data are presented as mean ± SD. * p value < 0.05 from Student’s t test (n ≥ 3). ns, not significant. (C) Flow cytometry detection of CSC-like CD44+/CD24− populations in 5637 and HT1197 cells. PE, phycoerythrin. APC, allophycocyanin.