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. 2021 Dec 31;23(1):470. doi: 10.3390/ijms23010470

Figure 3.

Figure 3

The expression of genes regulating autophagy is not changed by the additional lack of Hmox1 in the diaphragm of 6-week-old dystrophic mice. Analysis of mRNA levels of genes regulating autophagy in diaphragm muscle of WT, mdx, and mdx/Hmox1−/− animals. The expression of Becn1 (A), Map1lc3b (B), Sqstm1 (C), Atg5 (D), Atg7 (E), Lamp1 (F), and Ampka1 (G) was analyzed by qRT-PCR; n = 11–12/group. The protein level of AMPKα presented as a representative Western blot (H) and its densitometric analysis (I), n = 9–10/group. GAPDH was used as a loading control. Representative Western blot analysis of LC3-I/LC3-II protein (J) and densitometric analysis of LC3-II/LC3-I ratio (K), n = 5–7/group. GAPDH was used as a loading control. Results presented as mean ± SEM. * p < 0.05, ** p < 0.01 by one-way ANOVA with Tukey’s post hoc test.