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. 2021 Dec 31;23(1):470. doi: 10.3390/ijms23010470

Table 1.

The sequences of primers used for the determination of gene expression on mRNA level by qRT-PCR.

Gene Full Gene Name Primer Sequence
Ampka1 AMP-activated protein kinase F: 5′-TGTGACAAGCACATTTTCCAA-3′
R: 5′-CCGATCTCTGTGGAGTAGCA-3′
Atg5 Autophagy-related gene 5 F: 5′-CTGAAGATGGAGAGAAGAGG-3′
R: 5′-GGGGACAATGCTAATATGAAG-3′
Atg7 Autophagy-related gene 7 F: 5′-CTGTTCACCCAAAGTTCTTG-3′
R: 5′-TCTAAGAAGGAATGTGAGGAG-3′
Becn1 Beclin-1 F: 5′-CAATAATTTCAGACTGGGTCG-3′
R: 5′-ATTTGTCTGTCAGAGACTCC-3′
Bnip3 Bcl2/adenovirus E1B-19 kDa
interacting protein 3
F: 5′-ACCACAAGATACCAACAGAG-3′
R: 5′-AATCTTCCTCAGACAGAGTG-3′
Eef2 Elongation factor-2 F: 5′-AGAACATATTATTGCTGGCG-3′
R: 5′-CAACAGGGTCAGATTTCTTG-3′
Hmox1 Heme oxygenase-1 F: 5′-CCTCACTGGCAGGAAATCATC-3′
R: 5′-CCTCGTGGAGACGCTTTACATA-3′
Lamp1 Lysosomal-associated membrane protein 1 F: 5′-ATTGCAGTTTGGGATGAATG-3′
R: 5′-TTGCACTTGTATGAGTTTCC-3′
Map1lc3b Microtubule-associated
proteins 1A/1B light chain 3
F: 5′-GCTCATCAAGATAATCAGACG-3′
R: 5′-GCATAAACCATGTACAGGAAG-3′
Pink1 PTEN-induced kinase 1 F: 5′-ACTTACAGAAGATCCAGAGATG-3′
R: 5′-CTTCATAACGAGGAACAGTG-3′
Spp1 Secreted phosphoprotein 1 F: 5′-CCATCTCAGAAGCAGAATCTCCTT-3′
R: 5′-GGTCATGGCTTTCATTGGAATT-3′
Sqstm1 Sequestosome 1 F: 5′-AATGTGATCTGTGATGGTTG-3′
R: 5′-GAGAGAAGCTATCAGAGAGG-3′