Figure 3.

IL-4 causes LPS-induced neurotoxicity in the striatum in vivo. Animals unilaterally received intrastriatal injection of PBS (A) as a control, LPS (B), LPS+non-specific IgG (1 µg/µL; (C)), and LPS+IL-4NA (1 µg/µL; (D)). At 7 days after injection, animals were transcardially perfused, and brain tissues were processed for neuronal nuclei (NeuN) immunostaining and Nissl staining at 7 days post LPS injection. Scale bar, 25 µm. (E) Quantification of NeuN⁺ and Nissl⁺ cells in the LPS-injected striatum (Total area = 4.6 × 10⁵ μm²). *** p < 0.001 significantly different from PBS (control). ^### p < 0.001 significantly different from LPS mean ± SEM; n of animals = 5 to 6 in each group. ANOVA and Bonferroni analysis.