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Time course of 5 µM [14C]-MeHg-Cys uptake in BeWo cells (A). Uptake was carried out at 37 °C for time periods ranging from 5 to 60 min. Saturation kinetics for the transport of [14C]-MeHg-Cys (B) were assessed in BeWo cells (Inset: Eadie–Hofstee plot). Cells were incubated for 30 min with 5 µM [14C]-MeHg-Cys in the presence of unlabeled MeHg-Cys. Results are presented as mean ± SE. Data represent 3 experiments performed in triplicate.
