Figure 5.

Blockage of GPVI with the anti-GPVI nanobody 21 (Nb21) causes minor thrombus instability. Recalcified blood was perfused for 7 min on Horm collagen at 37 °C at arterial shear (1000 s⁻¹), and successively post-perfused for 10 min with rinse buffer and blocking anti-GPVI nanobody Nb21 (500 nM). (A) Representative images after perfusion of blood from 6 donors, taken during post-perfusion at time 0, 5 and 10 min. (B) Graphs showing extent of disaggregation, monitored from recorded brightfield images every min, and measured as morphological, contraction and multilayer scores [16]. Scale bar = 50 µm. Data are shown as mean ± s.e.m. * p < 0.05, ** p < 0.005 one-tailed Student’s paired t-test.