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. 2021 Dec 31;23(1):445. doi: 10.3390/ijms23010445

Figure 2.

Figure 2

Increased biglycan expression is required for the elevated proliferation and motility of CRC cells expressing L1. (A) Individual LS 174T CRC cells clones overexpressing a transfected biglycan cDNA were isolated (cl1, cl2, and cl3), and the level of biglycan in these cells was compared to that of L1-transfected cells (L1) and in cells transfected with an empty pcDNA3 plasmid. (B) Expression of endogenous biglycan was suppressed in LS 174T clones transfected with L1 (cl1, cl2, cl3, and cl4) using shRNA sequences that target biglycan (L1+shbiglycan). (C) The proliferation of CRC cells expressing L1 (L1), biglycan (biglycan cl1 and cl2), and the empty pcDNA3 plasmid were determined in the presence of 0.1% serum for five days. (D) The motility of the CRC cell clones described in (C) was determined by the “scratch wound” closure method, 24 h after introducing the wound in a confluent monolayer. (E) The proliferation of the CRC cell clones expressing L1 in which the endogenous biglycan levels were suppressed by shRNA (L1+shbiglycan cl1 and cl2) was compared to that of CRC cells expressing L1, or the empty pcDNA3 plasmid. (F) The motility of CRC cell cones described in (E) was determined by the “scratch wound” closure method, 24 h after introducing the wound. * p < 0.05.