Figure 4.

RvD2 counter-regulates histamine-stimulated increase in [Ca²⁺]_i in cultured conjunctival goblet cells derived from humans. Human goblet cells were preincubated with RvD2 (10⁻¹⁰–10⁻⁸ M) (a,b), RvD1 (10⁻¹⁰–10⁻⁸ M) (c,d), or vehicle for 30 min prior to the addition of histamine (10⁻⁵ M), and [Ca²⁺]_i was measured. The average [Ca²⁺]_i level over time was shown in (a,c); and change in peak [Ca²⁺]_i was calculated and shown in (b,c). Data are mean ± SEM from 4 humans. # Indicates a significant difference from stimulus alone. Arrow indicates addition of histamine.