Table 1.
List of primers used for RT-qPCR; primer sequences and amplicon lengths, with GUSB (G), HPRT1 (H), or ACTB (A) as reference genes, are provided.
| Gene | Sequence (5′→3′) | Amplicon Length (bp) |
|---|---|---|
| CCND1 | F: GAGGAGCTGCTGCAAATGG R: CGGCCAGGTTCCACTTGA |
58 (G) |
| ACTA2 | F: CGGGAGAAAATGACTCAAATTATGTT R: CATACATGGCTGGGACATTGAA |
62 (G) |
| PCG1-α | F: TGAGAGGGCCAAGCAAAG R: ATAAATCACACGGCGCTCTT |
64 (G) |
| TGFB3 | F: TATGTGATTGCCATCTTTGCC R: TGGACCTGCAGTGGCTAAACA |
54 (G) |
| COL1A2 | F: ACCACAGGGTGTTCAAGGTG R: CAGGACCAGGGAGACCAAAC |
149 (G) |
| COL6A3 | F: CCTAACCACATATGTTAGTGGAGGT R: GAATGTCTCGCTTGCTCTCTG |
71 (G) |
| GLUT1 | F: TTGTGGGCATGTGCTTCCAG R: ATCGAAGGTCCGGCCTTTAG |
134 (G) |
| HK2 | F: GGTGGACAGGATACGAGAAAACR: ACATCACATTTCGGAGCCAG | 141 (G) |
| GPI | F: AAATCGCCCAACCAACTC R: ATGATGCCCTGAACGAAG |
102 (G) |
| ALDOA | F: TTGTGGGCATCAAGGTAG R: TAGTCTCGCCATTTGTCC |
60 (G) |
| ALDOC | F: ATCGTCGTGGGCATCAAGG R: TTGGGCACAGCGTTCTGAG |
105 (G) |
| PKM2 | F: ATCGTCCTCACCAAGTCTGG R: GAAGATGCCACGGTACAGGT |
126 (A) |
| LDHA | F: GAG GTG ATC AAA CTC AAA GGC T R: CAT GGT GGA AAC TGG GTG C |
111 (G) |
| SLC1A5 | F: CTCGATTCGTTCCTGGATCTT R: GTTCCGGTGATATTCCTCTCTTC |
107 (A) |
| GLS1 | F: TCTACAGGATTGCGAACGTCT R: CTTTGTCTAGCATGACACCATCT |
100 (A) |
| GLUD1 | F: GGTCATCGAAGGCTACCG R: TCAGTGCTGTAACGGATACCTC |
75 (A) |
| GLUL | F: CCTGCTTGTATGCTGGAGTC R: GATCTCCCATGCTGATTCC |
105 (A) |
| GOT2 | F: GACCAAATTGGCATGTTCTGT R: CGGCCATCTTTTGTCATGTA |
95 (A) |
| ACTB | F: CTCTTCCAGCCTTCCTTCCT R: AGCACTGTGTTGGCGTACAG |
116 |
| HPRT1 | F: ATGGACTAATTATGGACAGGACTGAA R: CTCCCATCTCCTTCATCACATCT |
60 |
| GUSB | F: CAAGACAGTGGGCTGGTGAATTA R: CTTGAACAGGTTACTGCCCTTGAC |
55 |